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c iap 1  (R&D Systems)


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    Structured Review

    R&D Systems c iap 1
    C Iap 1, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 121 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+iap+2+antibody/Human+cIAP-1%2FHIAP-2+Antibody/pmc06834317-190-87-91
    Average 93 stars, based on 121 article reviews
    c iap 1 - by Bioz Stars, 2026-10
    93/100 stars

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    Western Blot:

    Article Title: N-(4-hydroxyphenyl) retinamide (4HPR) enhances TRAIL-mediated apoptosis through enhancement of a mitochondrial-dependent amplification loop in ovarian cancer cell lines.
    Article Snippet: Protein samples were boiled in an equal volume of sample buffer (20% glycerol, 4% SDS, 0.2% bromophenol blue, 125 mM Tris-HCl (pH 7.5), 640 mM b-mercaptoethanol), fractionated by 10–12% SDSPAGE and transferred to polyvinylidene fluoride membranes (Millipore, Bedford, MA, USA). .. Rabbit polyclonal anti-caspase 3 specific for the procaspase 3 or for the 18 kDa cleavage product (1 mg/ml; BD PharMingen, San Diego, CA, USA), anti-caspase 9 (1 mg/ml; Cell Signalling, Beverly, MA, USA), anti-PARP antibody (1 mg/ml; H-250, Santa Cruz Biotechnology), anti-TRAIL-R1 antibody (1mg/ml; BD PharMingen, San Diego, CA, , USA), anti-TRAIL-R2 antibody (1 mg/ml; Imgenex, San Diego, CA, USA), anti-TRAIL-R3 antibody (1 mg/ml; Affinity bioreagents, Golden, CO, USA), anti-TRAIL-R4 antibody (1 mg/ml; Oncogene Research Products, Cambridge, MA, USA), anti-IAP-1 antibody (1 mg/ ml; R&D Systems Inc., Minneapolis, MN, USA), anti-IAP-2 antibody (1.5 mg/ml; R&D Systems Inc.), anti-FLIP antibody (2 mg/ml; Calbiochem), anti-survivin antibody (1mg/ml; R&D Systems Inc.), anti-erk-2 antibody (1mg/ml; C-14, Santa Cruz Biotechnology), anti Smac/DIABLO (1mg/ml; Imgenex), mouse monoclonal anti-caspase 7 (1 mg/ml; BD PharMingen), anti-caspase 8 (1mg/ml; clone 5F7, Upstate Biotechnology, Lake Placid, NY, USA), anti-XIAP antibody (2 mg/ml; BD PharMingen), anti-cytochrome c antibody (1mg/ml; BD PharMingen), anti-FLAG (10 mg/ml; clone M5, Sigma Chemical Co.), anti-a-tubulin antibody (0.5 mg/ml; Sigma Chemical Co.), goat polyclonal anti-caspase 10 (1mg/ml; C-16, Santa Cruz Biotechnology, Santa Cruz, CA, USA), and anti-BID antibody (1mg/ml; C-20, Santa Cruz Biotechnology) were used for immunoblotting. .. Horseradish peroxidase-conjugated donkey anti-rabbit and anti-mouse (1 : 5000 dilution; Amersham Pharmacia Biotech Inc.) and rabbit anti-goat (1 : 10 000 dilution; Santa Cruz Biotechnology) antibodies were used to visualize immunoreactive proteins using SuperSignal detection reagent (Pierce, Rockford, IL, USA).



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